FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColSPL9_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColSPL9_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9983200
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT159470.1597383604455485TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28350.044.8139148
TGCCGTC28900.043.47749750
GCCGTCT30600.041.29125651
ATGCCGT32950.038.5576449
GTATGCC33250.038.10449247
CGTATGC38150.033.4855646
GTCACGA44750.028.4688429
AGTCACG46000.027.99957328
CATCTCG45850.027.32782741
TCACGAG50450.025.39108830
TCGTATG52800.024.39344445
CCGTCTT55200.023.65059352
CTCGTAT53000.023.50907544
CTGCTTG54150.023.3336859
CCAGTCA56650.023.04454826
CAGTCAC57800.022.52560827
CACGAGA58000.022.08586931
TCCAGTC62950.020.96065525
ATCTCGT61250.020.62825642
CGTCTTC65550.019.86289453
Produced by FastQC (version 0.11.5)