FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_ColSPL9_C24_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_ColSPL9_C24_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6107623
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11050.027.23946848
GCCGTCT12250.025.71395351
TGCCGTC12200.025.53245550
ATGCCGT15250.021.1144849
GTATGCC14800.020.57405947
CGTATGC15450.019.0288846
GTCACGA19200.016.04145829
AGTCACG19950.015.613831528
TCACGAG23450.014.32817230
TCGTATG23200.013.57741145
CATCTCG19100.013.56003341
CCGTCTT24350.013.22364752
CTCGTAT23100.013.18164744
CAGTCAC25200.012.49983727
TCCAGTC25700.012.12046425
CCAGTCA25450.012.10200426
CACGAGA27250.011.43104431
CGTCTTC30250.010.76019153
CGTCTGA29550.010.54131816
CTGCTTG28500.010.43863159
Produced by FastQC (version 0.11.5)