FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_C24SPL9_F1b_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_C24SPL9_F1b_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9407710
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10550.019.90487348
TGCCGTC11950.017.86580350
ATGCCGT14500.015.20663649
GCCGTCT15350.013.4526151
GTATGCC17350.012.91044147
CGTATGC22800.010.28491246
GTCACGA23800.09.558660529
AGTCACG24900.09.2769528
AGGGGGG14300.08.5693391
TCGTATG36000.08.06930645
CATCTCG28300.07.91505941
TCACGAG31250.07.391873430
CCGTCTT31500.07.222137552
CTCGTAT34300.06.836617544
CCAGTCA34750.06.748121726
CAGTCAC35950.06.52287227
CACGAGA36150.06.389932631
CTGCTTG38900.06.20825259
CGTCTTC44700.06.185609353
ATCTCGT39100.06.086852642
Produced by FastQC (version 0.11.5)