FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_C24SPL9_F1a_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_C24SPL9_F1a_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10174021
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC17500.029.79974750
TATGCCG17600.029.63028548
GCCGTCT19650.026.53921351
ATGCCGT21500.024.41827849
GTATGCC22100.024.23044447
GTCACGA29600.018.68236729
AGTCACG30150.017.7611328
CGTATGC30850.017.69830346
CATCTCG34550.014.79003341
TCACGAG36600.014.72672430
CCAGTCA38750.014.18059426
CTCGTAT39000.013.91013844
CAGTCAC39850.013.78916127
CCGTCTT40150.013.59888852
CACGAGA43200.012.88189931
TCCAGTC44450.012.12588125
TCGTATG45100.011.9510645
CGTCTGA47500.011.42095616
CTGCTTG47150.011.13474659
ATCTCGT48750.010.91272842
Produced by FastQC (version 0.11.5)