FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_C24SPL9_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_C24SPL9_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7898163
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT136480.17279967506368255TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC23550.046.0713350
GCCGTCT23800.045.58767751
TATGCCG23750.045.53648
ATGCCGT26750.040.55999849
GTATGCC27900.039.0136147
CGTATGC31100.034.77427746
AGTCACG35500.030.75999328
GTCACGA35100.030.61196929
TCACGAG39100.027.74884630
CATCTCG37750.027.35048941
CTCGTAT40350.027.14943544
TCGTATG43700.026.18944445
CAGTCAC43300.025.05727427
CCAGTCA43850.024.90261726
CACGAGA44250.024.67750731
CTGCTTG44500.024.61814359
CCGTCTT45950.024.45019152
TCCAGTC48200.022.87301825
CGAGATT51400.021.040633
CGTCTTC54100.020.8315453
Produced by FastQC (version 0.11.5)