FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_C24SPL9_C24_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_C24SPL9_C24_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5597774
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9600.024.4267548
TGCCGTC10250.022.53627850
GTATGCC10800.021.71266647
GCCGTCT11650.020.42890551
CGTATGC13400.017.49976246
ATGCCGT13750.016.79977249
GTCACGA17350.014.32257129
AGTCACG17100.013.713263528
CCAGTCA20650.011.694859526
CATCTCG20750.011.46972241
CTCGTAT22150.011.37682144
TCCAGTC21950.011.16167825
CCGTCTT22300.010.98639752
CAGTCAC21750.010.94247727
AGGGGGG4207.86029E-710.8365741
TCACGAG21800.010.75673330
TCGTATG22800.010.74546745
CACGAGA22650.010.66220131
CGTCTGA23600.010.23291116
CTGCTTG27800.09.19060459
Produced by FastQC (version 0.11.5)