FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_AtSG91_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_AtSG91_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023356
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT73890.3651853653039801TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT11100.049.18884351
TGCCGTC11300.048.3182450
ATGCCGT11750.046.16988849
TATGCCG12350.043.92681548
CGTATGC12550.043.50566546
GTATGCC12950.042.16186547
CCGTCTT13500.041.2219352
TCGTATG14400.037.91639745
CTCGTAT14350.037.5607144
CTGCTTG14450.036.57413559
GATCTCG16300.032.63780641
CGTCTTC17000.032.52918253
ATAAACT31750.031.1974035
TCTCGTA17150.031.0201943
CTTCTGC17600.030.6247856
ATCTCGT17150.030.6120342
ACTGTAG7550.028.7415189
CGATCTC18550.028.67904340
TTACTCG19300.028.28995935
TAAACTG16300.026.8409656
Produced by FastQC (version 0.11.5)