FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_AtSG141_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_AtSG141_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392700
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT75970.31750741839762614TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT11050.048.77752351
TATGCCG11650.046.565848
CGTATGC11800.045.3806546
TGCCGTC12150.044.3614550
ATGCCGT12150.044.0733949
GTATGCC12600.042.4993447
CTCGTAT13700.037.5541644
TCGTATG14650.036.55233445
CCGTCTT15450.034.8861952
GATCTCG15250.032.58965741
CTGCTTG16600.032.46937659
TTACTCG17500.031.39951135
CAGTCAC17450.031.28891227
CGATCTC16150.030.556840
TCTCGTA16800.029.99953543
CCAGTCA19050.028.47784426
CGTCTTC19200.028.4370653
CTTCTGC19700.027.1823256
ATCTCGT18900.026.66625442
ACTCCAG20600.026.50499723
Produced by FastQC (version 0.11.5)