FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_AtSG138_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_AtSG138_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333579
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT74100.3175379963566693TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11400.054.64870550
TATGCCG11750.053.61546748
GCCGTCT11850.052.5734451
ATGCCGT12000.052.20681449
CGTATGC12350.050.7272746
GTATGCC12800.049.2173247
TCGTATG13950.045.15997745
CTCGTAT14000.043.24967244
CCGTCTT15000.041.53301652
GATCTCG14400.041.3191341
CGATCTC14550.040.41206440
TCTCGTA15550.037.5881443
TTACTCG17050.037.56569735
CTGCTTG16550.037.4325459
CAGTCAC18150.035.48182327
CCAGTCA18350.035.095126
TCCAGTC18650.034.34216725
CTTCTGC19150.033.0806956
GTCACAT19850.032.7957229
CTCCAGT19950.032.6306324
Produced by FastQC (version 0.11.5)