FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_AtSG137_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_AtSG137_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161307
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT58010.2684024065068035TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8150.043.80298650
TATGCCG8400.043.74930248
GCCGTCT8300.043.01136851
ATGCCGT8550.042.16307449
CGTATGC9050.040.22035646
GTATGCC9100.039.99936747
CTCGTAT10800.033.379144
CCGTCTT11300.031.59241952
TCGTATG11900.030.5877545
CTGCTTG12250.029.71450259
GATCTCG12700.028.1097941
CGATCTC13800.025.86915440
TCTCGTA13900.025.68304643
CGTCTTC14850.024.74708453
TTACTCG15100.024.1055835
CCAGTCA15250.023.86847326
CTTCTGC15550.023.4079956
CTCCAGT15450.023.33296424
ATCTCGT15500.023.25769442
CAGTCAC15550.023.18291327
Produced by FastQC (version 0.11.5)