FastQCFastQC Report
Wed 12 Jun 2019
HL52FAFXY_n01_AtMCY2_Col_B_i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL52FAFXY_n01_AtMCY2_Col_B_i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518690
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCCG14050.047.82824731
ACTCCGG14200.046.58359532
ATCGGGG10400.046.1048742
CGGGGGT5000.040.59920544
CTCCGGA16300.040.36730633
GTCACTC16900.039.7625429
TCCGGAG16850.038.6342634
AATCGGG14100.038.47442241
CAGTCAC17700.037.9653627
TCACTCC18550.036.60305830
GCGGGGT4500.036.55556552
TCGGGGG7700.035.45385443
GAATCGG15650.035.3347740
CCAGTCA19150.035.090726
CGGGGTG4650.034.62366553
TCCAGTC19600.034.4636125
CCGGAGA19150.033.81134835
ACTCCAG20000.033.7743423
CTCCAGT20250.033.5302124
AGTCACT20450.033.20228228
Produced by FastQC (version 0.11.5)