FastQCFastQC Report
Wed 11 Oct 2017
HL327BGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL327BGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220976512
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1438350.021.4017525
GTTATAC1438650.021.3320033
ATACCGG1425650.021.0885986
GGACCAT824550.021.0589056
TACCGGT1423000.020.6288077
TGTTATA1676000.019.4611432
TGGACCA982300.019.1677285
TTATACC1595400.019.0532844
ACCGGTG1562150.018.7538078
GGCTTCA1560600.018.45273814
GGTATTC1369650.018.41457224
TTAGTCC469200.018.2234128
GGGTATT1372550.018.01468523
GGGGTAT1362850.017.97776622
TGGCTTC1614900.017.94794813
TTCAGGG1546350.017.92051117
GTGTTAT1875300.017.79961
CCGGTGG1620050.017.7637339
TGGCATA1392650.017.69971736
TCAGGGG1615100.017.42768718
Produced by FastQC (version 0.11.2)