FastQCFastQC Report
Wed 11 Oct 2017
HL327BGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL327BGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220976512
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG16530670.7480736233179389No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGAAC5020650.013.90622
CCTTCTT3765700.013.905051
CCTACAA2532500.013.8883341
AACTACC5039600.013.872516
AGAACTA5050050.013.8637654
GTCCTCT3699750.013.8635052
TTCCACC2265700.013.85905552
CCTCTGA3701350.013.8574624
TCCTCTG3701900.013.8548213
CCACACT2839200.013.8534312
TACTCCA2620050.013.8408433
ACACCAT2682900.013.8378033
TACACGT2459000.013.837752
CACGCTA4693350.013.8374184
AAGGTCC3911550.013.8372491
CTACTCC2623350.013.8359612
AGACCAG3289300.013.8354851
CTCCTAC2884600.013.8320244
CGCATCC2856300.013.8242265
GGTCCAA3956250.013.8222613
Produced by FastQC (version 0.11.2)