FastQCFastQC Report
Mon 23 Oct 2017
HL2YMBGX3_n01_tga1_30_n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2YMBGX3_n01_tga1_30_n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24985920
Sequences flagged as poor quality0
Sequence length75
%GC86

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG969758138.8121830214777No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307574512.309912942969481No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23455849.387623109335177No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6125902.451740820430066TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA792170.31704656062294284No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA604050.2417561570676605No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA396650.1587494076663977No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAA396200.1585693062332706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAA309840.1240058400891382No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG280370.11221119734634546RNA PCR Primer, Index 26 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG12867300.067.199341
CAGTCAC841650.065.8856627
CCAGTCA846700.065.6271426
ACGTCTG859700.065.6098115
CTCCAGT848500.065.5692324
AATCTCG705600.065.5457441
GTCACAT802800.065.5290529
CGTCTGA860250.065.5157416
AGTCACA825350.065.4941128
TCCAGTC848400.065.4875125
CACGTCT862650.065.4494314
ATGTCAG711800.065.4255234
TGTCAGA711000.065.4215135
CACACGT864650.065.41373412
ACACGTC865100.065.3876813
ACTCCAG852700.065.37572523
ACATGTC717300.065.2845532
GTCTGAA864700.065.2384217
GCACACG867400.065.2222611
CTGAACT862350.065.2201819
Produced by FastQC (version 0.11.2)