FastQCFastQC Report
Mon 23 Oct 2017
HL2YMBGX3_n01_tga1_30_k_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2YMBGX3_n01_tga1_30_k_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37869793
Sequences flagged as poor quality0
Sequence length75
%GC76

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG547592714.459880992747967No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32357728.544467090168673No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10643412.810527641384256TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA680880.17979501498727496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG448900.11853774854275016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG12260000.067.800891
TATGCCG1267100.066.16267448
CGTATGC1267800.066.1234246
CTCGTAT1257950.066.0570744
GTATGCC1271100.065.9626147
GTCCCGA1216400.065.9271636
GCCGTCT1273450.065.783351
CACCCGT1229900.065.75606531
TCGTATG1273700.065.7385945
CCGTCCC1220850.065.63882434
CCCGTCC1224150.065.62813633
CGTCCCG1220650.065.5902335
TCACCCG1235200.065.5465330
TCCCGAT1223350.065.5159737
ATGCCGT1279650.065.51379449
ACCCGTC1235900.065.4424132
AGTCACC1257900.065.4277228
CAGTCAC1288350.065.31116527
TCTCGTA1271200.065.2924743
CCGTCTT1287300.065.0621452