FastQCFastQC Report
Mon 23 Oct 2017
HL2YMBGX3_n01_input_bzip1_biotin_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2YMBGX3_n01_input_bzip1_biotin_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14798449
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG182571912.337232097769164RNA PCR Primer, Index 28 (100% over 43bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG997420.6740030661321332No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG533560.360551298315114RNA PCR Primer, Index 28 (100% over 43bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG195940.13240576765848908RNA PCR Primer, Index 28 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG158400.10703824434574191RNA PCR Primer, Index 28 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT2118100.065.20907640
TCTCGTA2118450.065.16247641
AAGATCT2124650.064.8310737
CGTATGC2168100.064.2238744
TATGCCG2167900.064.2106946
TGCCGTC2167250.064.2076748
GTATGCC2167950.064.2028545
ATGCCGT2165000.064.1995147
GCCGTCT2168750.064.1680449
TCGGAAG2218850.064.164633
TGCTTGA2174650.064.06042558
CCGTCTT2170700.064.05411550
GCTTGAA2175050.064.0145459
CTTGAAA2181800.063.8782660
CTCGTAT2165450.063.85829542
AGTCACC2205400.063.75399828
TCTGAAC2235900.063.6048318
ACGTCTG2240450.063.50815
TCACCAA2208350.063.49700530
ACACGTC2244150.063.4893713
Produced by FastQC (version 0.11.2)