FastQCFastQC Report
Mon 23 Oct 2017
HL2YMBGX3_n01_input_30_n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2YMBGX3_n01_input_30_n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3636625
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG271700.7471213006565153No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA43830.12052383734919053TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG38450.050.703731
GCACACG23550.050.535511
CACACGT24650.048.84013712
ACACGTC24600.048.7991813
ATGCCGT16500.048.29502549
GTATGCC16600.048.211947
GCCGTCT16650.047.85993251
TGCCGTC16900.047.15194750
TATGCCG17300.046.6599348
CACGTCT25600.046.62346314
CAGTCAC25200.046.40529627
ACGTCTG25700.046.17359515
CGTATGC17400.045.40049746
CGTCTGA26700.044.57344416
GTCACAG20900.044.0690229
ACTCCAG27400.043.3088123
CCAGTCA27150.043.0723226
TCCAGTC27400.042.6793225
GAGCACA28900.041.4190379
AGCACAC29300.041.32452410
Produced by FastQC (version 0.11.2)