FastQCFastQC Report
Mon 23 Oct 2017
HL2YMBGX3_n01_input_30_k_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2YMBGX3_n01_input_30_k_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3821549
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG395971.0361505243031033No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA49630.12986880450832897TruSeq Adapter, Index 14 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17800.041.4713711
ACGTCTG18100.040.40284315
CAGTCAC19350.038.14937227
AGGGGGG19800.037.8163721
CACGTCT19900.036.5749714
ACACGTC19800.036.4112613
CACACGT21050.034.90455612
CGTCTGA21000.034.33061616
TATGCCG14350.033.6539849
GTATGCC14950.032.99553348
CCAGTCA22100.032.7779426
AGCACAC22650.032.74348410
CGTATGC14800.031.93148447
TCCAGTC22700.031.75960225
CTCCAGT23600.031.13309124
ACTCCAG23350.031.02323223
GAGCACA24050.030.8374169
TGCCGTC15450.030.8109651
GAACTCC23900.030.74229621
ATGCCGT15650.030.63762550
Produced by FastQC (version 0.11.2)