FastQCFastQC Report
Mon 23 Oct 2017
HL2YMBGX3_n01_ev_biotin_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2YMBGX3_n01_ev_biotin_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14285839
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA878513461.495401145148TruSeq Adapter, Index 27 (97% over 44bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1739091.2173523725137878TruSeq Adapter, Index 27 (97% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1539901.0779205897532516No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG820770.5745339843183168TruSeq Adapter, Index 27 (97% over 44bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA685610.4799228102738663TruSeq Adapter, Index 27 (97% over 41bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG624280.4369921850582244TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA316270.22138706729090254TruSeq Adapter, Index 27 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG294210.20594520209838568TruSeq Adapter, Index 27 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA283660.19856026656887285TruSeq Adapter, Index 27 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGA247180.17302448949620672TruSeq Adapter, Index 27 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220860.15460065033632256No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA161460.11302101332655366TruSeq Adapter, Index 27 (97% over 41bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA157910.11053603502041427TruSeq Adapter, Index 27 (97% over 42bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA152540.1067770678361978TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA149390.10457208708567975TruSeq Adapter, Index 27 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9512300.068.10521
ATCGGAA9872000.067.6836242
TCGGAAG9897550.067.543263
CGGAAGA10033350.066.6699754
GCACACG10064950.066.5095111
AGCACAC10068000.066.50374610
CACACGT10066850.066.4924912
AGAGCAC10074850.066.486618
ACACGTC10068150.066.4839213
ACGTCTG10058500.066.4708815
GAGCACA10074600.066.460189
AAGAGCA10086150.066.4432457
CGTCTGA10062700.066.43764516
GTCTGAA10056100.066.43700417
TCTGAAC10057400.066.40748618
CACGTCT10071450.066.40082614
CTGAACT10051900.066.39818619
GAAGAGC10092950.066.3916856
TGAACTC10047300.066.3815520
GGAAGAG10090000.066.3584445
Produced by FastQC (version 0.11.2)