FastQCFastQC Report
Mon 23 Oct 2017
HL2YMBGX3_n01_bzip3_biotin_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2YMBGX3_n01_bzip3_biotin_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27117735
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG807816329.78922465316517RNA PCR Primer, Index 26 (100% over 43bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2777711.0243148994560203No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG2135360.7874403964785406RNA PCR Primer, Index 26 (100% over 43bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1552950.5726695094557123RNA PCR Primer, Index 26 (100% over 43bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG536960.19801063768784527RNA PCR Primer, Index 26 (97% over 44bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG510180.18813518164404217RNA PCR Primer, Index 26 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT9172400.066.66852642
ATCTCGT9170150.066.6634540
TCTCGTA9160300.066.6609441
GATCGGA9165800.066.659031
CATCTCG9150500.066.57928539
GCATCTC9172550.066.39516438
ATCGGAA9496400.066.318232
GAGCATC9159250.066.310836
TCGGAAG9514900.066.24453
AGCATCT9183300.066.21226537
TATGCCG9312000.066.1775546
CGTATGC9313950.066.1677744
TCGTATG9324950.066.1481743
ATGCCGT9305650.066.130447
GCCGTCT9310000.066.127349
TGCCGTC9313250.066.1179248
CCGTCTT9315700.066.0774650
GTATGCC9331750.066.0456245
CGTCTGA9558550.065.944616
ACACGTC9573450.065.93529513
Produced by FastQC (version 0.11.2)