FastQCFastQC Report
Mon 23 Oct 2017
HL2YMBGX3_n01_bzip1_biotin_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2YMBGX3_n01_bzip1_biotin_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27758649
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1800853764.87540874197444TruSeq Adapter, Index 25 (97% over 44bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3965221.428462890971387TruSeq Adapter, Index 25 (97% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2946381.0614277373513388No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1525760.5496521102305808TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1513650.545289505984243TruSeq Adapter, Index 25 (97% over 44bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1181250.4255430442598269TruSeq Adapter, Index 25 (97% over 41bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG581800.20959233282570777TruSeq Adapter, Index 25 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA568180.204685753978877TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA522210.18812514975062367TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGA444830.16024915333595666TruSeq Adapter, Index 25 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368350.13269738019310667No Hit
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA331000.11924211441270072TruSeq Adapter, Index 25 (97% over 42bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA312140.11244783562773535TruSeq Adapter, Index 25 (97% over 41bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA308060.11097802346216488TruSeq Adapter, Index 25 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303190.10922361531355505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG295370.10640647532954503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19337200.068.2429051
ATCGGAA20098100.067.8491442
TCGGAAG20149650.067.7352453
CGGAAGA20395450.066.9678044
GCACACG20457650.066.8224411
AGCACAC20466150.066.8213210
AGAGCAC20476300.066.8089148
ACACGTC20456700.066.80713
CACACGT20460700.066.80134612
ACGTCTG20437550.066.7956715
GAGCACA20476950.066.788269
CGTCTGA20444300.066.7746216
GTCTGAA20441700.066.7593217
AAGAGCA20503700.066.738657
CTGAACT20432050.066.7260219
CACGTCT20467300.066.72014614
TCTGAAC20448900.066.71860518
GAAGAGC20510050.066.718016
TATATCT20183500.066.67811639
TGAACTC20437550.066.6744820
Produced by FastQC (version 0.11.2)