FastQCFastQC Report
Mon 23 Oct 2017
HL2YMBGX3_n01_35s_bzip1_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2YMBGX3_n01_35s_bzip1_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775112
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA205880974.18832104794329TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1542075.556784735174652No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA227060.8182012113384974TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG165510.5964083611760534TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG128170.46185523323022637TruSeq Adapter, Index 7 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA92760.33425677954619487TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89840.3237346816993332No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA78490.28283543150690854TruSeq Adapter, Index 9 (97% over 35bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA76110.27425920107008295TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGA64020.23069339183427554TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA52720.18997431455018754TruSeq Adapter, Index 21 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG44550.16053406132797524TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA39020.14060693766593926TruSeq Adapter, Index 21 (97% over 37bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA38700.13945383105258452TruSeq Adapter, Index 7 (97% over 34bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA36220.13051725479908557TruSeq Adapter, Index 9 (96% over 33bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA31420.11322065559876501TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA30650.11044599281038027TruSeq Adapter, Index 7 (96% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28860.10399580269192737No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG28840.10392373352859272TruSeq Adapter, Index 7 (96% over 33bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28080.1011851053218753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2218550.068.347461
ATCGGAA2269750.067.804872
TCGGAAG2279800.067.65033
TATCGGA8950.067.4832461
CACGATT10650.067.0474931
CGGAAGA2309700.066.859674
GGAAGAG2318350.066.6622855
GAAGAGC2319300.066.6572956
AAGAGCA2319650.066.6442647
GAGCACA2317050.066.638659
AGAGCAC2318750.066.626998
AGCACAC2317500.066.6182610
GCACACG2317800.066.59476511
CACACGT2317500.066.58105512
ACACGTC2317850.066.5680213
CACGTCT2317300.066.5436414
ACGTCTG2317300.066.522815
CGTCTGA2317950.066.4981916
GTCTGAA2317000.066.4867417
TCTGAAC2316600.066.4773718
Produced by FastQC (version 0.11.2)