FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35549940
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG12152553.4184445880921315TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1374300.066.4029242
TCGTATG1376500.066.375643
TCTCGTA1375300.066.3510341
CGTATGC1382050.066.3191144
ATGCCGT1379700.066.2935147
CGATGTA1394100.066.0775534
TGCCGTC1384550.065.99522448
GCCGTCT1380900.065.9708249
TATCTCG1384150.065.9339239
CCGTCTT1384250.065.9329250
ATCTCGT1387100.065.8284240
TATGCCG1393250.065.7754346
CCGATGT1401750.065.7334333
ACCGATG1406400.065.6172332
CACCGAT1407750.065.6028331
CGTCTTC1388550.065.5804151
GTCACCG1419100.065.13222529
TCACCGA1429900.064.6302230
GTATGCC1425500.064.4009145
CGTCTGA1480150.063.0319616
Produced by FastQC (version 0.11.2)