FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29374354
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6479502.2058357436558436TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA750150.064.2262141
TCGTATG755800.064.1133143
CTCGTAT754600.064.0885742
CGTATGC756950.064.03435544
ATGCCGT760500.063.7221647
CGGCTAC760350.063.50074833
TGCCGTC764400.063.4065148
ATCTCGT759350.063.37395540
TATGCCG766000.063.26988646
CATCTCG760150.063.26230639
ACGGCTA770400.063.15597532
GCCGTCT766800.063.1326449
CCGTCTT767350.063.09285450
CGTCTTC774100.062.61951
TCACGGC784200.062.0107230
CACGGCT793100.061.36695531
GTCACGG795550.061.30238329
GTATGCC794350.061.06691745
AGTCACG802300.060.84408628
ACACGTC824600.060.35883713
Produced by FastQC (version 0.11.2)