FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28544148
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1881220.6590562801173817TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA230550.062.02643241
CTCGTAT229950.061.992842
CGTATGC232650.061.71637744
TCGTATG230600.061.54703543
ATGCCGT233600.061.30294447
TATCTCG237350.060.5867939
TGCCGTC238450.059.91190748
TATGCCG241250.059.74555646
ATCTCGT241550.059.54718840
GCCGTCT240250.059.0623149
CCGTCTT242700.058.82259850
CGTCTTC245500.057.8289951
GTATGCC271750.053.2042745
GCTTATC279550.052.6311536
CGTCTGA295950.050.95221716
ACACGTC302400.050.06868413
ACGTCTG302100.050.00526415
CACGTCT315750.047.91949514
ACTAGCT312150.047.8855132
TAGCTTA313300.047.2807234
Produced by FastQC (version 0.11.2)