FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33487953
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG12234913.653525791797426TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1494150.064.2097852
GATCGGA1483000.064.158541
TCGTATG1438100.063.92794443
TCTCGTA1426000.063.87726241
CTCGTAT1428850.063.87445442
GATCTCG1426200.063.70615439
CGTATGC1442800.063.7005744
TATGCCG1445700.063.570546
ATGCCGT1446250.063.5410847
ACGATCA1449800.063.4618732
TCGGAAG1506950.063.4552233
CGATCAG1445050.063.43140833
CACGATC1451500.063.39686631
ATCTCGT1434250.063.37207840
ACACGTC1476600.063.36132413
TCACGAT1456300.063.20222530
TGCCGTC1454400.063.17684648
CCGTCTT1454450.063.1732350
CGGAAGA1508750.063.157644
CGTCTGA1475750.063.11573816
Produced by FastQC (version 0.11.2)