FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39157095
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4932101.2595673912990737TruSeq Adapter, Index 8 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG627350.063.11357539
TCTCGTA622450.063.00694741
CTCGTAT622450.062.87336342
TCGTATG620200.062.82293343
CGTATGC633250.062.62875444
ATGCCGT623200.062.61004647
TGCCGTC625500.062.08309648
TATGCCG639700.061.8204146
GCCGTCT619050.061.67751749
ATCTCGT641150.061.6355640
CCGTCTT628300.061.41311350
CGTCTTC631700.060.6081151
CGTCTGA727600.059.10577816
GTATGCC685500.058.16199545
ACGTCTG740700.058.05910515
ACACGTC744150.057.9323113
CACGTCT762300.056.58975214
GCACACG780750.055.3308711
CACACGT784800.054.97089412
TCGCGTA17450.054.36834741
Produced by FastQC (version 0.11.2)