FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32779564
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1948280.5943581189792518TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT236750.061.2897442
TCGTATG237200.061.201943
TCTCGTA237700.060.90067741
CGTATGC240250.060.61179744
ATGCCGT240900.060.3624247
TGCCGTC244300.059.41018348
GCCGTCT246100.058.7939549
CCGTCTT248650.058.37216650
TATGCCG250600.058.20546346
ATCTCGT251650.057.51309640
CGTCTTC253700.057.251351
CATCTCG256650.056.38014239
GTATGCC282800.051.65067345
CGTCTGA332200.044.8631716
CTTAGGC332250.044.21905533
ACGTCTG337450.044.1953315
GCTTGAA332700.043.92532359
ACACGTC341750.043.80925813
TAGGCAT345300.042.30773535
AGGCATC346600.042.1189936
Produced by FastQC (version 0.11.2)