FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36783490
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4730931.286155826975635TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT546500.063.44342
TCGTATG550900.063.3921443
CGTATGC553150.063.14761744
TCTCGTA547200.063.1416941
ATGCCGT553250.063.0931547
CGATCTC545300.063.090738
TGCCGTC557650.062.62660648
GATCTCG550700.062.5440939
ACGATCT550500.062.43859537
CACGATC551050.062.36544436
TATGCCG560750.062.27406746
GCCGTCT562300.062.0913249
CCGTCTT565150.061.7797950
ATCTCGT560600.061.48240
CGTCTTC571150.061.17340551
TCACGAT569900.060.74413335
ATCACGA578350.059.994534
CATCACG581050.059.95918333
GTATGCC598950.058.3532245
CGTCTGA619500.057.16266316
Produced by FastQC (version 0.11.2)