FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32342025
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1581880.48910975735130996TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT204250.058.10363842
TCTCGTA204700.057.99347741
ATGCCGT206350.057.92975647
CGTATGC206800.057.7706944
TCGTATG207350.057.6506443
AATCTCG208750.056.77294539
TGCCGTC210750.056.6392548
TATGCCG213450.056.03550346
CCGTCTT215200.055.32495550
GCCGTCT215900.055.16061849
ATCTCGT215950.054.87931440
CGTCTTC219050.054.3692451
GTATGCC252200.047.54858845
CGTCTGA276700.045.00944516
ACGTCTG287450.043.32565715
GTAATCT275500.043.27002337
ACACGTC296250.042.08337413
GCTTGAA297300.040.40546859
CACGTCT309900.040.3090414
TGTAATC309650.038.62177736
Produced by FastQC (version 0.11.2)