FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33516531
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3808821.136400422824188TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT441200.064.6277542
TCGTATG441450.064.5823643
TCTCGTA442050.064.441941
CGTATGC448950.064.21062544
ATGCCGT448550.063.99907747
TGCCGTC451200.063.5246548
GCCGTCT451300.063.09059549
CCGTCTT453200.062.89563850
TATGCCG460350.062.60619746
ATCTCGT456550.062.46575540
CGTCTTC458700.062.0217151
CATCTCG461800.061.72590639
GTATGCC491900.058.8212645
CGTCTGA514550.057.1631816
ACGTCTG521850.056.38270615
ACACGTC525950.056.05334513
GATCATC521850.055.43424636
CACGTCT539550.054.62241414
GCTTGAA537300.053.36998759
CACACGT554950.053.1550912
Produced by FastQC (version 0.11.2)