FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32694237
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3889201.1895674457856287TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG452250.064.389943
CGTATGC452650.064.3481444
CTCGTAT452550.064.2951342
TCTCGTA452350.064.2783841
ATGCCGT453850.064.193247
CGCCAAT454200.063.83605233
TGCCGTC457750.063.57962448
GCCGTCT459100.063.34844249
TATCTCG458800.063.258139
CCGTCTT461100.063.08221450
TATGCCG464400.062.76498846
ATCTCGT464550.062.6155640
ACGCCAA466500.062.2782732
CGTCTTC467250.062.25230451
GTCACGC470700.061.8459629
TCACGCC482200.060.29265230
CACGCCA486950.059.72532731
AGTCACG493500.059.1080428
GTATGCC493250.059.10706745
CGTCTGA523100.056.2947816
Produced by FastQC (version 0.11.2)