FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31343679
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3291831.0502372743161388TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT385650.064.2485642
TCTCGTA385350.064.14709541
TCGTATG399550.063.9032543
ATGCCGT402900.063.7227847
CGTATGC403750.063.6571944
GATCTCG390500.063.15495339
TGCCGTC407650.062.9554948
GCCGTCT407500.062.62418749
TATGCCG410600.062.61250346
CCGTCTT409950.062.35230350
ATCTCGT397500.062.1372740
CGTCTTC413050.061.75168651
GTATGCC443100.058.24521345
AGTGATC467850.053.1832736
CGTCTGA493750.053.08751716
GCTTGAA490350.052.38010859
ACGTCTG501750.052.34321215
GTGATCT481500.051.50870537
ACACGTC511450.051.42312613
TGCTTGA510650.050.22447258
Produced by FastQC (version 0.11.2)