FastQCFastQC Report
Fri 27 Oct 2017
HL2NTBGX3_n01_ba100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NTBGX3_n01_ba100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31057459
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7767722.501080336288941TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA886700.065.2410541
CTCGTAT888300.065.2010142
TCGTATG899150.065.187443
CGTATGC899700.065.151844
ATGCCGT900100.065.0580147
AATCTCG891300.064.78413439
TGCCGTC903850.064.74280548
GCCGTCT906300.064.5566849
CCGTCTT906750.064.5413250
TATGCCG908050.064.5082546
ATCTCGT897600.064.4050640
CGTCTTC912450.064.1421151
GTATGCC936850.062.57174345
ACCAATC950700.060.61514336
CGTCTGA984400.060.1090716
ACGTCTG991450.059.7183815
GACCAAT978100.059.52387235
ACACGTC997550.059.4659913
GCTTGAA986850.059.44758259
ACTGACC988050.059.2841532
Produced by FastQC (version 0.11.2)