FastQCFastQC Report
Fri 6 Oct 2017
HL2NLBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NLBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328865303
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15331000.46617870174039No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT6215450.18899683071765097No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG5651900.1718606355988853No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG4032020.12260399510738293No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC3378340.10272716425788464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC2184100.018.8950843
TATACCG2290850.018.524155
ATACCGG2348600.018.3793266
TTATACC2347250.017.6472284
TACCGGT2291800.017.5994327
TCAGGGG2262200.017.21941418
GGTATTC2189550.017.16026724
GGACCAT1036500.017.1116146
GGGGGTA2262200.017.08870321
GGCTTCA2293550.016.91698514
TGTTATA2597500.016.8211672
ACCGGTG2420600.016.4873588
TTCAGGG2388850.016.3101817
TGGCATA2223750.015.93843936
GGGGTAT2370200.015.836322
GTCATAT1837850.015.6235161
TGGCTTC2481550.015.61877213
TGGACCA1271200.015.5837715
GCTTCAG2543550.015.51371315
CCGGTGG2532550.015.4035139
Produced by FastQC (version 0.11.2)