FastQCFastQC Report
Fri 6 Oct 2017
HL2NLBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NLBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328865303
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAC5291050.013.8796564
AATCCTT4878050.013.8545142
CCTACGT5289100.013.8504176
TCTCCTA5307300.013.8496963
TAGACCT6284900.013.8475784
TACACCT3683200.013.8463361
TCAGAGC5254000.013.8395741
TCCTTAC4874450.013.82576754
ACACCTT3690300.013.8239472
AGTCTCC5282850.013.822981
TCCTACG5307600.013.8183055
GAATCCT4886950.013.8171421
CCTCTCT2584500.013.8135874
CCTTACG4874900.013.8122485
CCTATAG3659000.013.810962
AGACCTT6291750.013.8081965
GTCTCCT5326000.013.8077522
CCACACT2810000.013.8071042
TCTCGAT5622000.013.8063476
ACACCAT4411250.013.8041883
Produced by FastQC (version 0.11.2)