FastQCFastQC Report
Wed 7 Dec 2016
HL2NLBCXX_l02n01_sd2_3_2016-12-01.3510000007294d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NLBCXX_l02n01_sd2_3_2016-12-01.3510000007294d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72400813
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC735528310.159116583400797RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG56064767.743664425425719Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC25434153.5129646955760014RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG23877793.298000258643505Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA11542061.5941892807198172RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA8957391.237194670728352RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC8368151.1558088443012373No Hit
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT7485761.0339331410546453RNA PCR Primer, Index 1 (100% over 25bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA6554800.905348949603646RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG4676690.6459444039668449No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC3448930.47636619771106714RNA PCR Primer, Index 1 (100% over 24bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG3376260.46632901760371115No Hit
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA2459400.3396923180959308No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA2370020.3273471528558664RNA PCR Primer, Index 1 (100% over 22bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG2315890.3198707174738494No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC2315500.3198168506754199No Hit
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA2230570.3080863194174353No Hit
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT2150560.29703533853963765No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG2071450.28610866565821574No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG2060710.284625256901466Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA1999940.2762317047461884No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA1904210.2630094775316957RNA PCR Primer, Index 1 (100% over 23bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC1847820.2552208909587797No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA1684710.23269213841562802RNA PCR Primer, Index 1 (100% over 22bp)
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT1617200.2233676574874926RNA PCR Primer, Index 9 (100% over 36bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG1581300.21840914963206282No Hit
ACTGAGCATTACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATT1544410.21331390298061984No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA1498960.2070363491636482RNA PCR Primer, Index 1 (100% over 23bp)
CTCATAATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAAT1490140.20581813079916655No Hit
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG1424250.19671740426450735No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1396760.19292048557521033RNA PCR Primer, Index 1 (100% over 30bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG1326070.18315678305988084No Hit
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG1290250.17820932480412893No Hit
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1237900.17097874301494376RNA PCR Primer, Index 1 (100% over 31bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA1208650.1669387331327343RNA PCR Primer, Index 7 (97% over 35bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG1197000.16532963517964916No Hit
GCACCGCCGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG1171180.161763376883627Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCC1127480.15572753305960807No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG1111740.15355352432299343No Hit
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG1105680.15271651714739723No Hit
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC1096010.15138089678633856RNA PCR Primer, Index 1 (100% over 26bp)
CGCGCAGCTACGCCGTCTGGGATAATGGCTGAAAGCCTTGGAATTCTCGGG1091980.15082427320256747No Hit
TCGGTCATAGTGTAATGGTTATCATTTAAGATGGAATTCTCGGGTGCCAAG1080310.14921241284956288No Hit
CTAGAGGAAGGAGAAGTCGTAACAAGGTAACGGTATGGAATTCTCGGGTGC1048000.1447497557796761No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC1047520.14468345818160908No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGATGGAATTCTCGGGTGCCAA1027460.14191277106239125No Hit
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG988320.1365067544200091No Hit
TAAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTC984250.13594460603639907No Hit
ATGACAGGTTAATCTTTGAATGCAAATTGCGCTAAACTTGGAATTCTCGGG983980.13590731363748637No Hit
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG974500.13459793607566256No Hit
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT960100.13260900813365176RNA PCR Primer, Index 1 (100% over 25bp)
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT939940.1298245090148366No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC892390.12325690320632172RNA PCR Primer, Index 1 (100% over 26bp)
CATGACAGGTTAATCTTTGAATGCAAATTGCGCTAAACTTGGAATTCTCGG884660.12218923563745065No Hit
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA867300.11979147250735982RNA PCR Primer, Index 1 (100% over 22bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG861890.11904424332914604No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG839560.11596002381906954No Hit
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC827660.11431639586699116RNA PCR Primer, Index 1 (100% over 24bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG814040.11243520152183926Illumina Small RNA Adapter 2 (100% over 21bp)
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA803170.11093383716561304RNA PCR Primer, Index 1 (100% over 23bp)
ACATCAGTACTGGGCTGGGCGACTTCCCGGGAACGTGCGGTGCTTGCTTCT791940.10938274961083655No Hit
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG771950.10662173089133681RNA PCR Primer, Index 6 (100% over 34bp)
TCCGACATCGTTCATCGGTTAGGATAATGGAATTCTCGGGTGCCAAGGAAC737160.10181653623143706RNA PCR Primer, Index 1 (100% over 24bp)
ATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGG728260.10058726826727761Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG728120.10056793146784139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC12265900.044.978451
CACCGCT12069800.044.8621982
TACATCT7889150.044.8269651
ACATCTT7900000.044.7626882
CATCTTT7903500.044.7499923
CCCCAAT979700.044.7323722
GCCCCAA993050.044.7200361
CTTTTTT7897550.044.7100336
ATCTTTT7914700.044.6863824
TCCGACA3408300.044.66111
TCTTTTT7923800.044.6583255
TCCGATA1087900.044.65451
CGCGATG7832250.044.61624516
TCGCGAT7834000.044.614915
TAATCGC7910250.044.61319412
AATCGCG7901350.044.60592313
TTAATCG7913600.044.60254711
ATCGCGA7849100.044.58037614
ACCGCTG12150450.044.5626643
TTTAATC7932150.044.50420410
Produced by FastQC (version 0.11.2)