FastQCFastQC Report
Wed 7 Dec 2016
HL2NLBCXX_l02n01_sd2_2_2016-12-01.35100000072916.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NLBCXX_l02n01_sd2_2_2016-12-01.35100000072916.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63711663
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG47183757.405826151485012Illumina Small RNA Adapter 2 (100% over 21bp)
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC24900573.9083220916710335RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC24298453.813815062400741RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG21575883.3864882792338977Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC8936781.4026913722217549No Hit
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA8918361.3998002218212386RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA7198471.1298512173508954RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG6517331.0229414353852293No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA6452341.0127407912739619RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT4952310.7773003821921898RNA PCR Primer, Index 1 (100% over 25bp)
ACTGAGCATTACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATT3595280.5643048432121447No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG3570740.5604531151541281No Hit
ACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCC3050170.4787459401271632No Hit
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT2834740.4449326648403448No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG2604870.4088529285446528No Hit
ATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGG2452580.3849499266719816Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG2385810.37446989886294446No Hit
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA2098580.32938710138518906No Hit
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA1873300.2940277983326224No Hit
AGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGGAA1773380.27834464154545774RNA PCR Primer, Index 1 (100% over 23bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG1667450.26171817238548617No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC1664990.261332057836883No Hit
CGCGCAGCTACGCCGTCTGGGATAATGGCTGAAAGCCTTGGAATTCTCGGG1603990.2517576726885939No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA1598910.2509603304500151RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG1540000.24171398571090508No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA1467730.23037069366718615RNA PCR Primer, Index 1 (100% over 23bp)
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1461190.2293441940135827RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA1449680.2275376174060941RNA PCR Primer, Index 1 (100% over 22bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC1417290.22245377584948614No Hit
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG1334690.20948911661590125No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA1243690.19520601746025684RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG1223370.19201664850594152Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG1185280.18603815128793608No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG1156790.18156644255228432No Hit
ATGGTGCGTAGATTCTGGGCGCGAGCATGCGCTTCATGGAATTCTCGGGTG1141950.17923719868997925No Hit
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG1130950.17751067022061565No Hit
ATGTTGGACCGATACGAATAATCATACTCAAAAGTGGAATTCTCGGGTGCC1123400.1763256438620979No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1047800.16445968456356258RNA PCR Primer, Index 1 (100% over 30bp)
GATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAG1018020.15978550112559453No Hit
ATGACAGGTTAATCTTTGAATGCAAATTGCGCTAAACTTGGAATTCTCGGG1003160.15745311812061788No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC1002680.15737777869650021No Hit
GCGGAGGAAAAGAAACTAACTAGGATTCCCTTAGTAAAGGTGGAATTCTCG992440.15577053764865625No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA935660.1468585116040685No Hit
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT889560.13962278774609918No Hit
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC859630.13492506073809438RNA PCR Primer, Index 1 (100% over 26bp)
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC837430.13144061237265148RNA PCR Primer, Index 1 (100% over 24bp)
GCGGGGAAAGAAGACCCTGTAGAGCTTTGGAATTCTCGGGTGCCAAGGAAC832270.13063071356338635RNA PCR Primer, Index 1 (100% over 24bp)
ATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGTG814620.12786042015572566No Hit
CTTGGGCTCTGGGGGAACTACGACCGCAAGGCTTGGAATTCTCGGGTGCCA793680.12457373777859165No Hit
CATGACAGGTTAATCTTTGAATGCAAATTGCGCTAAACTTGGAATTCTCGG789860.12397416152832175No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA784730.123168971433064RNA PCR Primer, Index 1 (100% over 23bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA780380.12248620790199748No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG765730.1201867858950723No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTTGGAATTCTCGGGTGCCAAGGAA759070.11914145138543944RNA PCR Primer, Index 1 (100% over 23bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG757670.11892171139842951No Hit
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG752240.11806943416309822No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGATGGAATTCTCGGGTGCCAA746430.11715751321700707No Hit
CCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGGAACTCC723220.11351453814664986RNA PCR Primer, Index 1 (100% over 27bp)
TCCCCATGGGCTTGTCCCGCATCAGTTCAAATCTGGTTCGTGTCGCCATGG713220.11194496681086476No Hit
TAAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTC692910.10875716742788522No Hit
CAATGAGAGCCATCGGGGGTAGATCTATCTTGGAATTCTCGGGTGCCAAGG692090.10862846257835085Illumina Small RNA Adapter 2 (100% over 21bp)
CGATCTCGGTCGAGAAGCATGGGTGTGACAGTACTACATGGAATTCTCGGG684730.107473258075213No Hit
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG683180.10722997451816632No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGTGGAATTCTCGGGTGCCAAGG678630.1065158195603841Illumina Small RNA Adapter 2 (100% over 21bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG677100.10627567514600898Illumina Small RNA Adapter 2 (100% over 21bp)
CTCATAATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAAT663260.1041033884172824No Hit
CTTTTGGTACTGTGGATAGGGGTACGGTTTTCCACCGTTGGAATTCTCGGG661520.10383028300485579No Hit
AAAACAGAGGGTGAAAAGTTCCTGAACTTGCTTGGAATTCTCGGGTGCCAA655580.10289795763139946No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG652570.10242551665932814No Hit
CCAACCGCAAGCTTACGATGGACTGGAATTCTCGGGTGCCAAGGAACTCCA646650.10149633042854336RNA PCR Primer, Index 1 (100% over 28bp)
ACAATGTTAGGATCAATAGGACTGCGAGCCTGAGAGGGTGCGCTACTCTTT638370.1001967253625133No Hit
TACTGAGCATTACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAAT637600.10007586836965786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC10262400.044.974091
CACCGCT10169650.044.871892
CCCCAAT590200.044.6844642
TCCGACA3000000.044.6838841
TCCGATA1059600.044.668381
TACATCT2711500.044.6049271
ACCGCTG10229800.044.604913
GCCCCAA595700.044.6006551
TAATCGC2701900.044.5797612
AATCGCG2695450.044.5520513
TTAATCG2704750.044.546111
TCGCGAT2669200.044.5417615
CGCGATG2668350.044.53149416
ATCGCGA2681850.044.50280414
CATCTTT2720850.044.4589423
CCGACAT2971900.044.455012
ACATCTT2721650.044.4345782
CTTTTTT2718350.044.428576
CTGTGAC193300.044.3603944
CGACATC2978450.044.3494153
Produced by FastQC (version 0.11.2)