FastQCFastQC Report
Wed 7 Dec 2016
HL2NLBCXX_l02n01_sd2_1_2016-12-01.35100000072909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NLBCXX_l02n01_sd2_1_2016-12-01.35100000072909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554039
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1322013.7197397102282785RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG1256613.535723721658654Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC1096553.0853628786853493No Hit
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC792022.2285067777815604RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG558851.5724363182283592Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG331580.9329666894482588No Hit
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA282260.7941949989856611RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA255180.71799999943726RNA PCR Primer, Index 1 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA200870.5651879453208026RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT183950.517580139103707RNA PCR Primer, Index 1 (100% over 25bp)
ACTGAGCATTACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATT171560.4827183944801956No Hit
ACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCC109740.3087754523796728No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG108780.30607430025388016No Hit
ATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGG91130.2564124929411298Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG87650.2466208164851314No Hit
CGTAGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA83260.23426867290989212RNA PCR Primer, Index 7 (100% over 40bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT82660.23258045283127168No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC73530.20689137063493115RNA PCR Primer, Index 1 (100% over 24bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA72880.20506246554975902RNA PCR Primer, Index 1 (100% over 22bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG70480.19830958523527736No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG69550.19569284411341575No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC68490.1927103219745197RNA PCR Primer, Index 7 (100% over 51bp)
AGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGGAA64070.18027376739534934RNA PCR Primer, Index 1 (100% over 23bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA58550.16474214267204157No Hit
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA58440.16443263565762783No Hit
GATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAG57430.1615907985252835No Hit
ATGGTGCGTAGATTCTGGGCGCGAGCATGCGCTTCATGGAATTCTCGGGTG56640.15936797542176662No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG56380.15863641338769777RNA PCR Primer, Index 7 (100% over 36bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC56160.1580173993588703No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG51050.14363939168928647No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA50970.14341429567880376RNA PCR Primer, Index 1 (100% over 23bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA49280.13865914245735628No Hit
CAATGAGAGCCATCGGGGGTAGATCTATCTTGGAATTCTCGGGTGCCAAGG48240.13573289432108088Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTTGGAATTCTCGGGTGCCAAGGAA47890.13474809927521897RNA PCR Primer, Index 1 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA47170.1327222351808745RNA PCR Primer, Index 1 (100% over 23bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC47150.1326659611782538No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG46660.13128724811404716Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTGGTACTGTGGATAGGGGTACGGTTTTCCACCGTTGGAATTCTCGGG42790.12039822860694552No Hit
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA42670.12006058459122142RNA PCR Primer, Index 7 (100% over 35bp)
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA42420.11935715955846292RNA PCR Primer, Index 1 (100% over 22bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG42100.11845677551653204No Hit
GGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA41750.11747198047067013RNA PCR Primer, Index 7 (100% over 37bp)
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG41240.11603699340384278No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG41180.11586817139598074No Hit
AAGGGTCGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC40490.11392671830556726RNA PCR Primer, Index 1 (100% over 31bp)
ATCACAAGGAATAGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC39710.11173203220336074RNA PCR Primer, Index 1 (100% over 33bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA39130.11010008612736101No Hit
CTAGAGGAAGGAGAAGTCGTAACAAGGTAACGGTATGGAATTCTCGGGTGC38950.10959362010377488No Hit
ATGTTGGACCGATACGAATAATCATACTCAAAAGTGGAATTCTCGGGTGCC37640.10590767293212032No Hit
GCGGGGAAAGAAGACCCTGTAGAGCTTTGGAATTCTCGGGTGCCAAGGAAC37510.1055418919150859RNA PCR Primer, Index 1 (100% over 24bp)
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT36070.10149016372639691RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTA351.2103192E-745.0121461
GTCCCAG4950.045.0121421
TTACAGG253.8917544E-545.001382
TGTCGAG600.045.00074838
CGAGACC207.0351956E-445.0001130
GCGTAGC253.8927246E-544.999485
GCTTAGC1850.044.9994775
GCGGTCC650.044.99884815
ATCGCGG950.044.99884814
GACGGGC1700.044.99884420
GATTACC1700.044.99884411
TCGCGGT900.044.99884415
CACCGCT310250.044.892592
TCCGACA85000.044.8003271
GCACCGC315200.044.769381
TCCGATA32200.044.7325671
CATCTTT145600.044.7077563
ACATCTT145900.044.6004072
ACCGCTG312450.044.5692983
TACATCT146000.044.5342751
Produced by FastQC (version 0.11.2)