FastQCFastQC Report
Wed 7 Dec 2016
HL2NLBCXX_l01n01_c37_3_2016-12-01.35100000072930.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NLBCXX_l01n01_c37_3_2016-12-01.35100000072930.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49663605
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC789680815.900593603706376RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG16727403.368140512554415Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG12292152.475082104893513Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC8918431.7957677458170827RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC8234481.658051202686555No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC7933181.5973830333098051RNA PCR Primer, Index 6 (100% over 51bp)
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG5243241.0557509870658806Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA4069710.8194552127256971No Hit
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA2958370.5956816868207614RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA2767510.5572511298766974RNA PCR Primer, Index 1 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA2275240.4581302545395164RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT1802650.36297203958512475RNA PCR Primer, Index 1 (100% over 25bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG1719490.3462273832115087No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA1575580.31725042916236146RNA PCR Primer, Index 6 (100% over 37bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT1489820.2999822505837021No Hit
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1407430.2833926373246566RNA PCR Primer, Index 1 (100% over 31bp)
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1393280.2805434684010555RNA PCR Primer, Index 1 (100% over 24bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG1387180.2793152047661462No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1318010.2653875005650516RNA PCR Primer, Index 1 (100% over 30bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG1175010.2365937792876695No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC1153470.23225659917357994RNA PCR Primer, Index 6 (100% over 36bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC1066980.21484143166811995RNA PCR Primer, Index 5 (97% over 35bp)
CATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA1051630.21175063711142195RNA PCR Primer, Index 6 (100% over 37bp)
CGTAGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA949070.1910996996694058RNA PCR Primer, Index 6 (100% over 40bp)
TTTTTTATGACTGACAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA931220.18750551837708923RNA PCR Primer, Index 1 (100% over 32bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA885490.17829756820915435No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA859710.1731066441914557RNA PCR Primer, Index 6 (100% over 37bp)
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG802870.16166164337043193RNA PCR Primer, Index 6 (100% over 34bp)
GTTAAAACTCACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC763110.15365578072715422RNA PCR Primer, Index 6 (100% over 36bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG733350.14766346502635078No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA679230.13676614897368003No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA671170.13514323013804574RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG646990.13027447363114295No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG646320.13013956598599719Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG643460.12956369156044953No Hit
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC612490.12332773667960674RNA PCR Primer, Index 1 (100% over 33bp)
ACATCAGTACTGGGCTGGGCGACTTCCCGGGAACGTGCGGTGCTTGCTTCT606670.1221558523590867No Hit
CATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGT594210.11964697286876375RNA PCR Primer, Index 1 (100% over 30bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA584570.11770591361621856RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC580900.1169669418883305No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC568800.11453055008793663RNA PCR Primer, Index 1 (100% over 26bp)
GCACTGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA566040.11397481113181374RNA PCR Primer, Index 1 (100% over 22bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC562940.11335061157964671No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG545680.1098752295569361No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA527650.106244804419655No Hit
TTTCGATGAGGATTACCCCGACGGGCGGTCTGAAATATGACGATAGTCGGA518780.10445878828168031No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTTGGAATTCTCGGGTGCCAAGGAA510200.10273116500503739RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC3709450.044.9461331
TACATCT8339650.044.8459631
CACCGCT3676850.044.768742
CATCTTT8387100.044.712473
ACATCTT8387850.044.7001572
GCACTGC671850.044.6934281
ATCTTTT8412150.044.585124
CTTTTTT8405050.044.553276
TCTTTTT8431200.044.490925
GCCCCAA340000.044.468871
CGACGGG99050.044.4307319
TACCCCG99250.044.40920314
TCCGACA1557300.044.3728871
CACTGCT671600.044.3166122
CGCGATG8372000.044.3133116
TCGCGAT8374450.044.31189715
TGTGACG421550.044.3113025
TAATCGC8447500.044.3037212
TTAATCG8454200.044.2821811
AATCGCG8435000.044.27334613
Produced by FastQC (version 0.11.2)