FastQCFastQC Report
Wed 7 Dec 2016
HL2NLBCXX_l01n01_c37_2_2016-12-01.35100000072923.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NLBCXX_l01n01_c37_2_2016-12-01.35100000072923.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45097626
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC641409714.222693230016143RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG19744484.378163941489958Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG14182663.144879510952528Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC9370542.0778344296881612No Hit
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC9064032.0098685460738RNA PCR Primer, Index 1 (100% over 24bp)
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG6058361.3433877871974902Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA3567310.7910194651931346No Hit
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA3466530.7686723908704196RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA3319430.7360542659163477RNA PCR Primer, Index 1 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA2363560.5240985412402861RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG2310750.512388390466496No Hit
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT2288040.5073526486737905RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC2133030.472980551127015RNA PCR Primer, Index 5 (100% over 51bp)
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC2068670.4587092899302504RNA PCR Primer, Index 1 (100% over 24bp)
ACATCAGTACTGGGCTGGGCGACTTCCCGGGAACGTGCGGTGCTTGCTTCT1728980.3833860345553444No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA1710200.37922173552993677No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG1597680.35427141996343664No Hit
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1516630.3362992987701836RNA PCR Primer, Index 1 (100% over 31bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT1362000.3020114628650298No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1212840.26893655111690357RNA PCR Primer, Index 1 (100% over 30bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG1099070.24370905909770063No Hit
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA1077000.23881523164877902No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG1033370.2291406647436386RNA PCR Primer, Index 5 (100% over 37bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA958690.21258103475335932RNA PCR Primer, Index 1 (100% over 22bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG892160.19782859523470261No Hit
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG876280.19430734557956553No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG851750.18886803487172474Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC831970.18448199468415477RNA PCR Primer, Index 5 (100% over 35bp)
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA824610.18284997973064038RNA PCR Primer, Index 1 (100% over 34bp)
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC777540.17241262322766168RNA PCR Primer, Index 1 (100% over 26bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA752520.16686465935036138RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTATGACTGACAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA745640.16533908015468488RNA PCR Primer, Index 1 (100% over 32bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC722670.1602456856598172No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA679440.15065981521954172RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC667390.1479878342154862RNA PCR Primer, Index 1 (100% over 33bp)
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA651510.14446658456034914RNA PCR Primer, Index 5 (100% over 36bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC638560.14159503651034758No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG626690.13896296891548127RNA PCR Primer, Index 5 (100% over 37bp)
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG588880.13057893557412534No Hit
CATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGT583850.12946357752844906RNA PCR Primer, Index 1 (100% over 30bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG550450.1220574227122288No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG530750.11768912181763182No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA524960.11640524048871219RNA PCR Primer, Index 1 (100% over 23bp)
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA524810.11637197931438786RNA PCR Primer, Index 1 (100% over 22bp)
CATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG519050.11509475022033311RNA PCR Primer, Index 5 (100% over 37bp)
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG504750.11192385160141245No Hit
TTTCGATGAGGATTACCCCGACGGGCGGTCTGAAATATGACGATAGTCGGA473680.10503435369303032No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG470500.10432921679735425No Hit
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA464910.10308968370086709RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA462000.10244441691897486No Hit
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG461550.10234463339600182No Hit
AGGGGCTTAGCTCAATGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCC458810.10173706261167716RNA PCR Primer, Index 1 (100% over 27bp)
CTCATAATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAAT455120.10091883772329834No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC454950.10088114172573076RNA PCR Primer, Index 5 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC4230850.044.9698331
TACATCT6777500.044.828951
CACCGCT4174050.044.7895742
GCACTGC765600.044.718951
CATCTTT6809500.044.716133
ACATCTT6812650.044.6888962
TACCCCG82900.044.6185814
CTTTTTT6819650.044.5776336
GCCCCAA438250.044.5763971
ATCTTTT6832300.044.57184
TAATCGC6822600.044.53817712
TCGCGAT6761100.044.5373215
CGCGATG6760200.044.53193316
TTAATCG6826950.044.52232411
TCCGACA1818100.044.5152781
AATCGCG6813500.044.5048713
TCTTTTT6845250.044.4931955
ATCGCGA6777800.044.48814
TTTAATC6838050.044.45860310
TGTGACG365800.044.421865
Produced by FastQC (version 0.11.2)