FastQCFastQC Report
Wed 7 Dec 2016
HL2NLBCXX_l01n01_c37_1_2016-12-01.351000000728fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2NLBCXX_l01n01_c37_1_2016-12-01.351000000728fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67086662
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC827374212.332916489420803RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG22666903.3787491170748667Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG17768522.6485920554520956Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC13605212.028005209142765RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC12803671.9085269140384418No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC8537161.2725569801043313RNA PCR Primer, Index 4 (100% over 51bp)
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG6720721.0017967505970113Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA5649650.8421420639470779No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA4753740.7085968891998233RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA4064820.605905835648821RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA3529220.5260688033636254RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT2676080.398898964446912RNA PCR Primer, Index 1 (100% over 25bp)
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2454170.36582085422583704RNA PCR Primer, Index 1 (100% over 31bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT2253420.3358968732115484No Hit
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG2202730.32834097484236136No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2160770.3220863783623636RNA PCR Primer, Index 1 (100% over 30bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG2045120.30484748220145463No Hit
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG1808030.26950662711464163RNA PCR Primer, Index 2 (97% over 35bp)
ACATCAGTACTGGGCTGGGCGACTTCCCGGGAACGTGCGGTGCTTGCTTCT1708330.25464525273295013No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC1657540.24707444827110342RNA PCR Primer, Index 4 (100% over 37bp)
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1642770.24487281838527006RNA PCR Primer, Index 1 (100% over 24bp)
CATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC1587950.23670129838923867RNA PCR Primer, Index 4 (100% over 37bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG1547020.23060023466363552No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA1537820.22922887413894585RNA PCR Primer, Index 2 (97% over 36bp)
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1523720.2271271150739323RNA PCR Primer, Index 3 (100% over 34bp)
CGTAGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA1386850.20672514605064116RNA PCR Primer, Index 4 (100% over 40bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA1381270.20589338608023156No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA1360280.2027645972309667No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG1317440.19637882713556387No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA1223660.1823998934393248RNA PCR Primer, Index 1 (100% over 23bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA1101070.16412651444783463No Hit
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1099220.16385075173363076RNA PCR Primer, Index 1 (100% over 33bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTTGGAATTCTCGGGTGCCAAGGAA1040320.15507106315708477RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTATGACTGACAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA989110.1474376531060675RNA PCR Primer, Index 1 (100% over 32bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG946740.14112194164616507No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG917350.1367410410134879No Hit
GTTAAAACTCACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA898230.1338909960969589RNA PCR Primer, Index 2 (97% over 36bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG864590.1288765865262457No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG858950.1280358829002403Illumina Small RNA Adapter 2 (100% over 21bp)
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC850260.12674054344811492RNA PCR Primer, Index 1 (100% over 26bp)
CATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGT840770.12532595525471218RNA PCR Primer, Index 1 (100% over 30bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC824980.1229722832237502No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA824200.12285601570100477RNA PCR Primer, Index 1 (100% over 22bp)
AGGGGCTTAGCTCAATGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCC816400.12169334047355046RNA PCR Primer, Index 1 (100% over 27bp)
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG779680.11621982324891943No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA778760.11608268719645047RNA PCR Primer, Index 1 (100% over 23bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC777880.11595151358104537RNA PCR Primer, Index 4 (100% over 37bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGTGGAATTCTCGGGT777280.11586207702508734No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA766830.11430439034215177RNA PCR Primer, Index 1 (100% over 22bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG686080.1022677205194678No Hit
CAAGTCTCTAAGCAATGGATGTCTTGGCTGGAATTCTCGGGTGCCAAGGAA680430.1014255262841964RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC5228600.044.9577141
TACATCT8839100.044.8231961
CACCGCT5180900.044.7620122
CATCTTT8887150.044.6906243
ACATCTT8887850.044.679512
GCACTGC849200.044.617491
TACCCCG120550.044.60661714
CGACGGG120700.044.56982419
ATCTTTT8924800.044.5028154
TCCGACA2343700.044.4707831
CTTTTTT8916450.044.4706046
GCCCCAA436450.044.4503061
TCTTTTT8941600.044.4271555
TCCGATA794850.044.4205971
TGTGACG582300.044.370045
TAATCGC8949500.044.27678312
CGCGATG8849100.044.27393316
TCGCGAT8852350.044.27216715
TTAATCG8955650.044.2609511
CACTGCT847350.044.2538532
Produced by FastQC (version 0.11.2)