FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64118907
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1109750.17307687418938691No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG884520.13794994977066594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG114500.035.671381
TGGGGGG297750.022.0303541
GAGACCG18850.012.24889331
CGAGACC18850.012.06408530
ATGGGGG51200.010.6820371
ATGCCGT42700.09.905474
CCGAGAC25100.09.6171829
AGACCGA24050.09.16515632
CGACGCT11400.08.89160717
AGCGGTT35650.07.647195311
ACCGATC22550.07.292877734
GATCTCG22800.07.212270337
CGGTTCA41700.06.78923513
CGATCTC26500.06.46910636
GGAATGC50300.06.048255423
CGCAGGC17253.0377123E-105.896291370
GCTTGAA89250.05.874292416
CCGATCT28600.05.75012735
GTCACGA55100.05.587318429
GGTTCAG52450.05.5310114
Produced by FastQC (version 0.11.5)