FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-vec..ph..bZIP63_Col..h_B_q2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-vec..ph..bZIP63_Col..h_B_q2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107556
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG6780.6303692959946446TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT3540.3291308713600357TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG1630.15154896054148537TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG1330.1236565138160586TruSeq Adapter, Index 6 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGT200.00693500152.540
CGGGGGT552.2537279E-950.9090944
ATCGGGG1500.042.042
CACTCCG1700.041.1764731
ACTCCGG1700.041.1764732
GTCACTC1750.040.029
CAGTCAC1850.039.72972527
TCGGGGG1600.039.37543
AATCGGG1650.038.1818241
CCAGTCA1900.036.84210626
AGTCACT1950.035.89743428
CTCCAGT1950.035.89743424
TCCAGTC1950.035.89743425
ACTCCAG1950.035.89743423
TCCGGAG2000.035.034
TCACTCC2000.035.030
GGGGGTA400.002409728735.062
GAATCGG1800.034.99999640
CTCCGGA2050.034.14634333
CGGAGAA2000.033.2536
Produced by FastQC (version 0.11.5)