FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-vec..ph..bZIP63_Col..h_B_q1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-vec..ph..bZIP63_Col..h_B_q1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171185
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG9200.5374302655022345TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT4410.25761602944183193TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG2830.16531822297514387TruSeq Adapter, Index 6 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2210.1291000963869498No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG2070.12092180973800273TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG2150.045.5813942
AATCGGG2300.044.13043241
TCGGGGG2250.042.043
GAATCGG2450.041.42857440
TCCGGAG2950.037.96610334
AGAATCG2800.037.539
ACTCCGG3000.037.33333232
GTCACTC3100.037.25806429
CGGGGGG1600.037.18750444
CACTCCG3050.036.7213131
TCACTCC3050.036.7213130
CGGAGAA3050.035.5737736
TCCAGTC3300.034.99999625
CCGGAGA3250.034.46153635
AGTCACT3400.033.9705928
CAGTCAC3400.033.9705927
CCAGTCA3500.033.026
CTCCGGA3400.032.94117733
GGAGAAT3650.032.60273737
CGGGGGT1108.967618E-1031.8181844
Produced by FastQC (version 0.11.5)