FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-vec..ph-bZIP53_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-vec..ph-bZIP53_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334809
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9150.018.36038448
ATGCCGT9550.017.59136449
GTATGCC9700.016.95851147
CGTATGC10950.016.94038846
CGCTCAT11250.015.86661534
CCGCTCA11100.015.45039833
TGCCGTC12100.015.04109950
ACCGCTC12150.014.11501832
TCGTATG12850.013.89084445
GCCGTCT11750.012.80832151
CACCGCT13850.012.38248731
CTCGTAT14550.011.30567444
CCGTCTT15700.010.47755152
CTCCAGT19550.09.66759924
TCACCGC18550.09.62249930
TCTCGTA18700.09.17098743
TATCTCG18750.09.14653141
GCTCATT20200.09.009871535
ACTCCAG20150.08.85873623
GAACTCC22300.08.47540721
Produced by FastQC (version 0.11.5)