FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-vec..ph-bZIP44_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-vec..ph-bZIP44_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12148286
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20650.014.0675645
GTATGCC26200.012.15628444
ATGCCGT23650.012.13510246
CGTATGC24500.011.99984743
CGCTCAT26950.011.55829531
GCCGTCT23250.010.83852748
CCGCTCA31600.010.522017530
TGCCGTC26100.010.45959447
TCGTATG32150.09.14451842
ACCGCTC37250.08.92606229
CACCGCT36400.08.65373728
CTCGTAT33700.08.308541
TCACCGC45400.07.47787927
TATCTCG47550.06.69815538
CCGTCTT43100.06.658820649
GCTCATT52250.06.5645132
TCTCGTA49050.06.207872440
CGCCCTA7700.00151546145.909015721
ACTCCAG58600.05.79341820
GTCACCG59000.05.51687926
Produced by FastQC (version 0.11.5)