FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-vec..ph-bZIP2_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-vec..ph-bZIP2_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314834
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC14890.11324623488592475TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3700.027.43186646
TATGCCG3850.027.27216545
CGCTCAT4950.025.4540231
CCGCTCA5300.024.43345830
CGTATGC4450.024.3815243
GTATGCC4950.021.91873744
TGCCGTC4800.021.87454847
ACCGCTC6150.019.91828729
TCGTATG5800.019.91338342
CACCGCT6450.019.5344828
CGCCTGG900.00527673119.4447828
CTCGTAT5750.018.86917541
GCCGTCT5050.018.71248448
TCACCGC6800.018.01433427
TATCTCG6850.016.35002738
CCGTCTT6150.015.9346349
GTCACCG8150.015.45980426
TCTCGTA6900.015.21707840
TCCAGTC8550.015.14588622
ATCTCGT7700.014.99969139
Produced by FastQC (version 0.11.5)