FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-vec..ph-bZIP1_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-vec..ph-bZIP1_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4247484
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10950.016.94018648
GTATGCC11650.016.52316344
ATGCCGT11800.015.71991849
CGCTCAT12250.015.71423534
TGCCGTC12300.015.36544247
CGTATGC12550.015.05935943
CCGCTCA13250.014.79240530
GCCGTCT12450.014.05584851
CTCGTAT13850.013.64584444
CACCGCT15800.012.84790928
ACCGCTC15100.012.74845329
TCGTATG14900.012.68422545
TCACCGC19650.011.04309227
TCTCGTA18450.011.00241743
TATCTCG19050.010.65588341
CCGTCTT17450.010.63008952
CAGTCAC22600.010.06645824
ACTCCAG22050.09.52389120
CTCCAGT21600.09.39822921
TCCAGTC21850.09.29069722
Produced by FastQC (version 0.11.5)