FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-vec..ph-bZIP11_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-vec..ph-bZIP11_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17830212
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31700.016.89240345
ATGCCGT36650.014.70638846
GCCGTCT36450.014.01892148
TGCCGTC39150.013.76728347
CGTATGC41850.013.21359243
GTATGCC42450.012.77948444
CTCGTAT51800.010.81059341
TCGTATG53800.010.343657542
CGCTCAT55950.010.25903931
CCGCTCA62550.09.34441430
ACCGCTC69600.08.498486529
CCGTCTT66650.08.24439449
TCTCGTA71050.07.980134540
TATCTCG72700.07.895323838
CACCGCT76700.07.3467428
TCACCGC86750.07.0201327
GCTCATT90800.07.015336532
ATCTCGT89350.06.97245939
CTGCTTG75350.06.22416156
GTCACCG98650.06.137909426
Produced by FastQC (version 0.11.5)